Methylocella tundrae sp. nov., a novel methanotrophic bacterium from acidic tundra peatlands.

Abstract

A novel species, Methylocella tundrae, is proposed for three methanotrophic strains (T4T, TCh1 and TY1) isolated from acidic Sphagnum tundra peatlands. These strains are aerobic, Gram-negative, non-motile, dinitrogen-fixing rods that possess a soluble methane monooxygenase and utilize the serine pathway for carbon assimilation. Strains T4T, TCh1 and TY1 are moderately acidophilic organisms capable of growth between pH 4.2 and 7.5 (optimum 5.5-6.0) and between 5 and 30 degrees C (optimum 15 degrees C). The major phospholipid fatty acid is 18:1omega7c. The DNA G+C content of strain T4T is 63.3 mol%. The three strains possess almost identical 16S rRNA gene sequences and are most closely related to two previously identified species of Methylocella, Methylocella palustris (97% similarity) and Methylocella silvestris (97.5% similarity). DNA-DNA hybridization values of strain T4T with Methylocella palustris KT and Methylocella silvestris BL2T were respectively 27 and 36%. Thus, the tundra strains represent a novel species, for which the name Methylocella tundrae sp. nov. is proposed. Strain T4T (=DSM 15673T=NCIMB 13949T) is the type strain.

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@article{Dedysh2004MethylocellaTS, title={Methylocella tundrae sp. nov., a novel methanotrophic bacterium from acidic tundra peatlands.}, author={Svetlana N. Dedysh and Yulia Y Berestovskaya and Lina V Vasylieva and Svetlana E Belova and Valentina N. Khmelenina and Natalia E. Suzina and Yuri Aleksandrovich Trotsenko and Werner Liesack and George A. Zavarzin}, journal={International journal of systematic and evolutionary microbiology}, year={2004}, volume={54 Pt 1}, pages={151-6} }