Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.

@article{Herman1996MethylationspecificPA,
  title={Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.},
  author={James Gordon Herman and Jeremy R. Graff and Sanna Myöhänen and Barry D. Nelkin and Stephen B. Baylin},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1996},
  volume={93 18},
  pages={
          9821-6
        }
}
  • J. Herman, J. Graff, S. Baylin
  • Published 3 September 1996
  • Biology
  • Proceedings of the National Academy of Sciences of the United States of America
Precise mapping of DNA methylation patterns in CpG islands has become essential for understanding diverse biological processes such as the regulation of imprinted genes, X chromosome inactivation, and tumor suppressor gene silencing in human cancer. We describe a new method, MSP (methylation-specific PCR), which can rapidly assess the methylation status of virtually any group of CpG sites within a CpG island, independent of the use of methylation-sensitive restriction enzymes. This assay… 

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