Methylation in Physarum DNA

@article{Reilly1980MethylationIP,
  title={Methylation in Physarum DNA},
  author={J. G. Reilly and Richard Braun and C. A. Thomas},
  journal={FEBS Letters},
  year={1980},
  volume={116}
}
The biological function of methylated bases in eukaryotic DNA is not known. An attractive hypothesis suggests a correlation between gene activity and state of methylation [l-3]. The major modified base in eukaryotes is S-methylcytosine (MeC) which is primarily found in the doublet CpG [4,5]. Changes in the relative amount of MeC occur during the development of sea urchin [6] and tissue differences are known in several mammals [4]. The pattern of modified bases in chicken globin DNA [7… 
Eukaryotic DNA methylation
  • D. Cooper
  • Biology, Medicine
    Human Genetics
  • 2004
TLDR
The introduction of artificially methylated DNA sequences into individual eukaryotic cells by microinjection or transformation may provide the means to elucidate questions in the regulation of gene expression in these organisms.
Domains of DNA methylation in eukaryotes : studies on their structure and function
TLDR
Although the frequent occurrence of differentially methylated multisequence families precluded an assessment as to whether all transcribed sea urchin gene sequences reside in the unmethylated compartments, results obtained are consistent with this hypothesis.
25 DNA Methylation
TLDR
This chapter focuses on DNA methylation, its nature, distribution, analysis, specificity, and biological function and presents other DNA modifications known to occur among prokaryotes and eukaryotes.
Deoxyribonucleic acid methylation and chromatin organization in Tetrahymena thermophila
Deoxyribonucleic acid (DNA) of the transcriptionally active macronucleus of Tetrahymena thermophila is methylated at the N6 position of adenine to produce methyladenine (MeAde); approximately 1 in
Deoxyribonucleic acid methylation and chromatin organization in Tetrahymena thermophila.
TLDR
Pulse-chase studies showed that labeled MeAde remains preferentially in linker DNA during subsequent rounds of DNA replication; i.e., there is little, if any, movement of nucleosomes during chromatin replication, implying that nucleosome may be phased with respect to DNA sequence.
Methylation of mouse ribosomal RNA genes.
TLDR
The rDNA methylation level might be correlated directly with the number of rDNA genes and there are discrete hypomethylation sites at similar sites for both Hpa II and Hha I and show a tissue-specific pattern.
5-Methylcytosine in eukaryotic DNA.
TLDR
Methylation of eukaryotic DNA has been proposed to function in many ways, including control of transcription, maintenance of chromosome structure, repair of DNA, establishment of preferred sites for mutation, oncogenic transformation, and, in certain systems, protection of DNA against enzymatic degradation.
Resistance of DNA from filamentous and unicellular cyanobacteria to restriction endonuclease cleavage.
TLDR
The use of isoschizomers demonstrates the presence of a methylase in the filamentous strains analogous to the dam enzyme of Escherichia coli, and Nostoc PCC 73102, which lacks detectable sequence-specific endonucleases, is shown to possess extensive DNA modification.
Structure of the central spacer region of extrachromosomal ribosomal DNA in Physarum polycephalum.
TLDR
A model to explain the evolutionary origin of the several palindromic axes in the Physarum rDNA spacer is proposed and some of the CpG sequences in the spacer resist cleavage by certain restriction endonucleases and thus appear to be methylated.
DNA methylation in mouse cells in culture as measured by restriction enzymes.
TLDR
Southern blot hybridization using satellite DNA showed that these sequences followed a pattern of modification similar to that of total DNA, and total DNA methylation was estimated from the weight average lengths.
...
1
2
...

References

SHOWING 1-10 OF 33 REFERENCES
Specific DNA methylation sites in the vicinity of the chicken β-globin genes
TLDR
This technique is used to provide a correlation between DNA methylation and the activity of the chicken β-globin genes and it is shown that the overall content of 5-methylcytosine in DNA does not vary significantly between different tissues of the same organism.
Methylation of parental and progeny DNA strands in Physarum polycephalum.
TLDR
Continuing methylation of parental DNA has been shown, by density shift experiments and by the conversion of prelabeled DNA-cytosine to DNA-5-methylcytOSine, which once formed was found to be stable.
Rare Bases in Animal DNA
TLDR
This work has shown that 6-MeAde and certain N2-methylated derivatives of guanine have been recently discovered in appreciable amounts in bovine and human sperm DNA.
Methylation of DNA in developing sea urchin embryos.
TLDR
The data suggest that DNA 5-methylcytosine is synthesized at the polymer level in developing sea urchin embryos as it is known to occur in other organisms.
The distribution of modified and non‐modified CG doublets in BHK‐21 cell DNA
TLDR
The present study examines the distribution of 5MC in relatively long DNA sequences of baby hamster kidney fibroblast cells (BHK-21/C13) and indicates preferential methylation of (C + G)rich and rapidly-reannealing BHK-2 l/C 13 DNA, these findings are compared with human and mouse cell DNA.
Methylation of the deoxyribonucleic acid of Physarum polycephalum at various periods during the mitotic cycle.
Abstract The methylation of major nuclear DNA-cytosine was found to occur throughout the mitotic cycle of Physarum polycephalum, during the period of DNA synthesis (S), as well as during the
Direct detection of methylated cytosine in DNA by use of the restriction enzyme MspI.
TLDR
The results reveal a symmetric methylation of both strands at this sequence in calf thymus DNA, in contrast to the CCGG sequence in other eukaryotic DNAs from organisms like Neurospora, Drosophila and Herpes virus, which proved to be undermethylated at this sequences.
X inactivation, differentiation, and DNA methylation.
TLDR
A model based on DNA methylation is proposed to explain the initiation and maintenance of mammalian X inactivation and certain aspects of other permanent events in eukaryotic cell differentiation using sequence-specific DNA methylases that methylate unmethylated sites with great difficulty but easily methylate half-methylated sites.
Large scale isolation of ribosomal DNA from giant surface cultures of Physarum polycephalum
TLDR
A new method for growing Physarum polycephalum in very large surface cultures and an improved procedure for rapidly isolating nucleoli as well as rDNA are reported.
A deoxyribonuclease of Diplococcus pneumoniae specific for methylated DNA.
TLDR
A deoxyribonuclease specific for methylated DNA was isolated from Diplococcus pneumoniae and it is suggested that this pair of enzymes plays a role in some unknown control process, which would involve a large fraction of the specific base sequences that are methylated in E. coli DNA and are present but notmethylated in DNA from other sources.
...
1
2
3
4
...