Methylation defects of imprinted genes in human testicular spermatozoa.

  title={Methylation defects of imprinted genes in human testicular spermatozoa.},
  author={C Joana Marques and T{\^a}nia Francisco and Sonia M. Sousa and Filipa Abreu Gomes de Carvalho and Alberto Barros and M{\'a}rio Sousa},
  journal={Fertility and sterility},
  volume={94 2},
OBJECTIVE To study the methylation imprinting marks of two oppositely imprinted genes, H19 and MEST/PEG1, in human testicular spermatozoa from azoospermic patients with different etiologies. Testicular spermatozoa are often used in intracytoplasmic sperm injection for treatment of male factor infertility, but the imprinting status of these cells is currently unknown. DESIGN Experimental prospective study. SETTING University research laboratory and private in vitro fertilization (IVF) clinic… CONTINUE READING