Methyl groups of thymine bases are important for nucleic acid recognition by DtxR.

@article{Chen2000MethylGO,
  title={Methyl groups of thymine bases are important for nucleic acid recognition by DtxR.},
  author={Christopher S. Chen and Abraham White and Julie Love and John A Murphy and Dagmar Ringe},
  journal={Biochemistry},
  year={2000},
  volume={39 34},
  pages={10397-407}
}
The expression of diphtheria toxin is controlled by the diphtheria toxin repressor (DtxR). Under conditions of high iron concentration, DtxR binds the tox operator to inhibit transcription. To study how DNA binding specificity is achieved by this repressor, we solved the crystal structure of the nickel(II) activated DtxR(C102D) mutant complexed with a 43mer DNA duplex containing the DtxR consensus binding sequence. Structural analysis of this complex and comparison with a previously determined… CONTINUE READING