Methyl allyl ether formation in plants: novel S-adenosyl l-methionine:coniferyl alcohol 9-O-methyltransferase from suspension cultures of three Linum species

Abstract

A novel 41 kDa methyltransferase displaying high regiospecificity towards the allylic hydroxyl moiety of coniferyl alcohol was cloned from suspension cultures of Linum nodiflorum L. and expressed in E. coli. The apparent K m for coniferyl alcohol is 7.23 μM with a V max of 707.5 pkat mg−1 protein at 30°C, whereas the K m for the co-substrate S-adenosyl-l… (More)
DOI: 10.1007/s11103-007-9151-1

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