• DOI:10.1007/978-1-4757-5241-0_21
Corpus ID: 23078388

Methotrexate polyglutamates in cultured human cells.

@article{Rosenblatt1983MethotrexatePI,
  title={Methotrexate polyglutamates in cultured human cells.},
  author={D. Rosenblatt and V. Whitehead},
  journal={Advances in experimental medicine and biology},
  year={1983},
  volume={163},
  pages={
          275-85
        }
}

D. Rosenblatt, V. Whitehead

Published 1983

Chemistry, Medicine

Advances in experimental medicine and biology

Cultured human fibroblasts accumulated methotrexate polyglutamates to levels far in excess of the dihydrofolate reductase binding capacity. After four days in methotrexate-free medium the intracellular drug level dropped by 70% but nearly 80% of the remaining methotrexate was in the form of polyglutamates. Reduced folates prevented the accumulation of polyglutamates and the effects of methotrexate on deoxyuridine incorporation into DNA and cell growth if present along with methotrexate from the… CONTINUE READING

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