Methotrexate metabolism in mutant Chinese hamster ovary cells lacking dihydrofolate reductase.

@article{Joannon1987MethotrexateMI,
  title={Methotrexate metabolism in mutant Chinese hamster ovary cells lacking dihydrofolate reductase.},
  author={P Joannon and V. Michael Whitehead and David S. Rosenblatt and M. J. Vuchich and Denise Beaulieu},
  journal={Biochemical pharmacology},
  year={1987},
  volume={36 7},
  pages={1091-7}
}
To study the influence of the level of dihydrofolate reductase (DHFR) on methotrexate (MTX) metabolism, the formation of methotrexate polyglutamates (MTXPGs) and the retention of the drug were examined in Chinese hamster ovary cells (DUKXB11) lacking DHFR and in control cells (CHO-UTC). Both cells accumulated MTXPGs poorly. After a 24-hr incubation with 1.0… CONTINUE READING