Methotrexate inhibition of CCRF-CEM cultures of human lymphoblasts.

@article{Roberts1975MethotrexateIO,
  title={Methotrexate inhibition of CCRF-CEM cultures of human lymphoblasts.},
  author={D. Roberts and E. V. Warmath},
  journal={European journal of cancer},
  year={1975},
  volume={11 11},
  pages={
          771-82
        }
}
  • D. Roberts, E. V. Warmath
  • Published 1975
  • Biology, Medicine
  • European journal of cancer
  • Abstract The biochemical basis for methotrexate (MTX) inhibition of human lymphoblasts was examined with the CCRF-CEM cell line. Methotrexate inhibition of growth by cultures of this human lymphoblast cell line was prevented by a combination of hypoxanthine, thymidine and deoxycytidine and was reversed competitively by folinic acid. Within 5 min MTX inhibited the incorporation of isotopically-labeled deoxyuridine, hypoxanthine and glycine into DNA, although thymidine incorporation continued… CONTINUE READING
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