Methotrexate induced differentiation in colon cancer cells is primarily due to purine deprivation

@article{Singh2006MethotrexateID,
  title={Methotrexate induced differentiation in colon cancer cells is primarily due to purine deprivation},
  author={Ravinder Kaur Singh and A A Fouladi-Nashta and D Li and Nigel Halliday and David A. Barrett and Kevin D. Sinclair},
  journal={Journal of Cellular Biochemistry},
  year={2006},
  volume={99}
}
The folate antagonist methotrexate (MTX) inhibits synthesis of tetrahydrofolate (THF), pyrimidines and purines, and induces differentiation in several cell types. At 1 µM, MTX reduced proliferation and induced differentiation in HT29 colon cancer cells; the latter effect was augmented (P < 0.001) by thymidine (100 µM) but was reversed (P < 0.001) by the purines, hypoxanthine (Hx; 100 µM) and adenosine (100 µM). In contrast 5‐fluoro‐uracil (5‐FU), a specific thymidylate synthase (TS) inhibitor… Expand
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