Methotrexate differentially affects growth of suspension and adherent cells.

@article{Kimura2004MethotrexateDA,
  title={Methotrexate differentially affects growth of suspension and adherent cells.},
  author={Elza Kimura and Kazuhiro Nishimura and Kaori Sakata and Seiji Oga and Keiko Kashiwagi and Kazuei Igarashi},
  journal={The international journal of biochemistry \& cell biology},
  year={2004},
  volume={36 5},
  pages={
          814-25
        }
}
The effects of low concentrations of methotrexate (MTX) on the growth of suspension (FM3A, 2B4 and THP-1) and adherent (NIH3T3 and V79) cells were compared. The concentration of methotrexate to cause the inhibition of cell growth was lower in suspension cells than in adherent cells. The IC(50) for FM3A, 2B4, THP-1, NIH3T3 and V79 cells were 3.5, 5, 9, 30 and 50 nM, respectively. The inhibition of cell growth was reversed completely by tetrahydrofolate and was fully or significantly reversed by… Expand
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