Methotrexate and sulfasalazine promote adenosine release by a mechanism that requires ecto-5'-nucleotidase-mediated conversion of adenine nucleotides.

@article{Morabito1998MethotrexateAS,
  title={Methotrexate and sulfasalazine promote adenosine release by a mechanism that requires ecto-5'-nucleotidase-mediated conversion of adenine nucleotides.},
  author={L. Morabito and Mar{\'i}a Carmen Montesinos and David Schreibman and Liliana Balter and Linda F. Thompson and Regina Resta and G Carlin and M A Huie and Bruce N. Cronstein},
  journal={The Journal of clinical investigation},
  year={1998},
  volume={101 2},
  pages={
          295-300
        }
}
We and others have shown that an increased extracellular concentration of adenosine mediates the antiinflammatory effects of methotrexate and sulfasalazine both in vitro and in vivo, but the mechanism by which these drugs increase extracellular adenosine remains unclear. The results of the experiments reported here provide three distinct lines of evidence that adenosine results from the ecto-5'-nucleotidase- mediated conversion of adenine nucleotides to adenosine. First, pretreatment of a human… Expand
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