Methotrexate and 7-hydroxymethotrexate: serum level monitoring by high-performance liquid chromatography.

Abstract

We have developed a reverse-phase high-performance liquid chromatographic assay with ultraviolet detection at 303 nm for methotrexate (MTX) and 7-hydroxymethotrexate (7-OH-MTX) in serum, plasma, and cerebrospinal fluid. The procedure involves the concentration of MTX and 7-OH-MTX in the aqueous phase by an n-butanol-ether extraction, resulting in a… (More)

Topics

Figures and Tables

Sorry, we couldn't extract any figures or tables for this paper.

Slides referencing similar topics