Methotrexate Promotes Platelet Apoptosis via JNK-Mediated Mitochondrial Damage: Alleviation by N-Acetylcysteine and N-Acetylcysteine Amide

@article{Paul2015MethotrexatePP,
  title={Methotrexate Promotes Platelet Apoptosis via JNK-Mediated Mitochondrial Damage: Alleviation by N-Acetylcysteine and N-Acetylcysteine Amide},
  author={Manoj Paul and Mahadevappa Hemshekhar and Ram Mohan Thushara and Mahalingam S. Sundaram and Somanathapura K. NaveenKumar and Shivanna Naveen and Sannaningaiah Devaraja and Kumar Somyajit and Robert West and Basappa and Siddaiah Chandra Nayaka and Uzma I. Zakai and Ganesh Nagaraju and Kanchugarakoppal S. Rangappa and Kempaiah Kemparaju and Kesturu S. Girish},
  journal={PLoS ONE},
  year={2015},
  volume={10}
}
Thrombocytopenia in methotrexate (MTX)-treated cancer and rheumatoid arthritis (RA) patients connotes the interference of MTX with platelets. Hence, it seemed appealing to appraise the effect of MTX on platelets. Thereby, the mechanism of action of MTX on platelets was dissected. MTX (10 μM) induced activation of pro-apoptotic proteins Bid, Bax and Bad through JNK phosphorylation leading to ΔΨm dissipation, cytochrome c release and caspase activation, culminating in apoptosis. The use of… Expand
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