Methotrexate: pentose cycle and oxidative stress

@article{Babiak1998MethotrexatePC,
  title={Methotrexate: pentose cycle and oxidative stress},
  author={R. Babiak and Annibal P. Campello and Eva Gunilla Skare Carnieri and M B M Oliveira},
  journal={Cell Biochemistry and Function},
  year={1998},
  volume={16}
}
The effect of methotrexate (MTX) and leucovorin (LCV) on pentose cycle enzymes and the activity of enzymes involved in enzyme defence mechanisms against ROS in HeLa cells, were studied. The effect of MTX was also investigated on the cellular levels of glutathione. MTX inhibited the activity of glucose‐6‐phosphate and 6‐phosphogluconate dehydrogenases. The activities of glutathione reductase and γ‐glutamylcysteine synthetase were also inhibited by the drug. No effect was observed on the… Expand
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