Methods to study degradation of ruminant feeds

@article{Mohamed2008MethodsTS,
  title={Methods to study degradation of ruminant feeds},
  author={Ruba A. M. Mohamed and Abdul Shakoor Chaudhry},
  journal={Nutrition Research Reviews},
  year={2008},
  volume={21},
  pages={68 - 81}
}
Ruman degradation is crucial in the supply of dietary nutrients to meet the nutrient demands of the anaerobic microbes and body tissues of ruminant animals. Therefore, it is essential to study the dynamics of rumen degradation of various feeds before their potential use to formulate nutritious diets for ruminant animals. Amongst many methods that have been used in the past, the in sacco method has been the most effective method to study rumen degradation. However, this method is undesirable due… 
Using fistulated sheep to compare in sacco and in vitro rumen degradation of selected feeds
Despite its beneficial role in almost all feed evaluation systems, the in sacco method is inconsistent and labour intensive in estimating the degradation of ruminant feeds. This study compared a
Assessment of the effectiveness of rumen liquor from slaughtered cattle as inoculum for estimation of in Vitro digestibility
TLDR
The obtained in vitro results using rumen liquor from fistulated and slaughtered cattle from all institutions were regressed against those determined by in vivo technique to derive prediction equations.
Monitoring digestibility of forages for herbivores: a new application for an old approach
TLDR
An in vitro method using a two-stage procedure using purified enzymes to measure digestibility of forages for wild herbivores was tested and validated using in sacco and in vivo met...
Invited review: Nitrogen in ruminant nutrition: A review of measurement techniques.
TLDR
Methods to study ruminant N metabolism have been developed over 150 yr of animal nutrition research, but many of them are laborious and impractical for application on a large number of animals and results can be variable, especially the methods based on measurements of digesta or blood flow.
Comparison of in vitro digestibility using slaughtered and fistulated cattle as sources of inoculum
TLDR
Rumen liquour from slaughtered cattle can successfully replace use of fistulated cattle as the source of inoculum and significantly increased the overall INVDMD with dietary crude protein levels.
Prediction of In Situ Ruminal Degradability of Forages in Buffaloes Using the In Vitro Gas Production Technique
Two experiments, namely the situ nylon bag technique and the in vitro gas production technique, were carried out to determine the correlations between the in situ ruminal degradability and the in
Effect of exogenous fibrolytic enzymes on fibre and protein digestion in ruminant animals
TLDR
The aim and objectives of the study are to evaluate ruminant feeds and investigate the mode of action of EFE and its implications for animal welfare and nutritional implications.
The development of an intraruminal nylon bag technique using non-fistulated animals to assess the rumen degradability of dietary plant materials.
TLDR
Ruminal degradation data obtained for tested forages were in accordance with those expected from the conventional degradability technique using fistulated animals, with no significant differences in the asymptotic values of degradation curves between bag shape or anti-regurgitation device.
In Sacco Degradation of Sorghum in Buffalo ’ s Rumen with Supplementation of BIOS-K 2 Probiotic by In Sacco
Degradation of Sorghum in Buffalo’s Rumen with Supplementation of BIOSK2 Probiotic by In Sacco. The level of ruminantia feed digestibility cauld be improved by utilizing of probiotic supplements. The
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References

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TLDR
This methodology offers a better way to simulate the rumen environment within a given feeding regimen, however, its usefulness will be dependent on the standardization of inherent variables associated with its conduct.
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TLDR
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TLDR
It was concluded that cattle faeces shows potential as an alternative to rumen liquor collected from rumen-fistulated sheep for use in the in vitro digestibility assay of forages.
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    Journal of the science of food and agriculture
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TLDR
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TLDR
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Summary A method is proposed for estimating the percentage of dietary protein that is degraded by microbial action in the rumen when protein supplement is added to a specified ration. The potential
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SUMMARY The artificial fibre bag technique was evaluated for assessing the proportions of dietary D. M. and N which disappear in the rumen. The most important factor determining the variability in
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SUMMARY A rapid method for measuring gas production during incubation of feedingstuffs with rumen liquor in vitro is described. Gas production in 24 h from 200 mg feed dry matter was well correlated
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TLDR
The protease preparation was used for the determination of the rates of feed protein degradation in vitro by incubating at pH 6.8 in 0.1 M potassium phosphate buffer and measuring the rate of amino acid (and ammonia) production by the ninhydrin method.
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