Methods to characterize the effect of DNA-modifying compounds on nucleosomal DNA.

@article{Subramanian2010MethodsTC,
  title={Methods to characterize the effect of DNA-modifying compounds on nucleosomal DNA.},
  author={Vidya Subramanian and Robert Michael Williams and Dale L. Boger and Karolin Luger},
  journal={Methods in molecular biology},
  year={2010},
  volume={613},
  pages={
          173-92
        }
}
Eukaryotic DNA forms a complex with an equal mass of proteins to form chromatin. To fully understand the action of DNA-reactive antitumor antibiotics in the cell, their effect must be studied in a chromatin context. In particular, it is of interest to investigate how the distortion of DNA, in the context of a nucleosome, affects the action of drugs with either monoalkylation or crosslinking activity, and how modified DNA is assembled into chromatin. Here, we present experimental approaches that… CONTINUE READING

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