Methods to Study Viruses

  title={Methods to Study Viruses},
  author={Susan L. Payne},
  pages={37 - 52}
  • S. Payne
  • Published 1 September 2017
  • Biology
  • Viruses

Towards a Quantitative Single Particle Characterization by Super Resolution Microscopy: From Virus Structures to Antivirals Design

The use of super-resolution microscopy (SRM) to study all steps involved in the viral infection and antiviral design is reviewed to inspire future assays to unveil the viral mechanism of emerging viruses and further develop successful antivirals against them.

Rapid high-throughput compatible label-free virus particle quantification method based on time-resolved luminescence

The Protein-Probe method is developed, a simple and cost-effective time-resolved luminescence-based method for virus particle quantification based on the recognition of the virus particles by an external Eu3+-peptide probe, providing results on virus count in minutes.

Inactivation of viruses on surfaces by infrared techniques

Choosing a cellular model to study SARS-CoV-2

This review revisits basic concepts of virology and presents the currently available in vitro models, their advantages and disadvantages, and the known consequences of each choice to help scientists choose the right cellular models for SARS-CoV-2.

In vitro effects of bufotenine against RNA and DNA viruses

The findings indicate that the antiviral activity of bufotenine is somewhat linked to the particular infectious dose used and the genetic lineage of the virus, although the mechanisms of its effects remain undetermined.

Recent Diagnostic Techniques for COVID-19

To contain SARS-CoV-2 ongoing pandemic and lower the global health pressure, it’s important to develop, produce & globally distributed most recent, rapid, widely available, cost-effective& point of care diagnostic test for COVID-19.

SARS-CoV-2 Antibody Neutralization Assay Platforms Based on Epitopes Sources: Live Virus, Pseudovirus, and Recombinant S Glycoprotein RBD

The current neutralization assay platforms used to evaluate nAbs, their techniques, advantages, and limitations are discussed, including neutralization assays based on the live virus, pseudovirus, and neutralization Assays utilizing recombinant SARS-CoV-2 S glycoprotein receptor binding site, receptor-binding domain.



Hepatitis C virus Cell-to-cell Spread Assay.

An assay for detecting HCV cell-to-cell transmission, using a non-growing cell culture system that avoids confounding effects of cell growth and may be modified to assess the effects of inhibitors and/or specific cellular genes on cell- to-cell spread of HCV.

Hepatitis C virus cell-to-cell spread assay. Bio-Protocol

  • 2014