Methods of single-molecule fluorescence spectroscopy and microscopy

@article{Moerner2003MethodsOS,
  title={Methods of single-molecule fluorescence spectroscopy and microscopy},
  author={W. Moerner and D. P. Fromm},
  journal={Review of Scientific Instruments},
  year={2003},
  volume={74},
  pages={3597-3619}
}
Optical spectroscopy at the ultimate limit of a single molecule has grown over the past dozen years into a powerful technique for exploring the individual nanoscale behavior of molecules in complex local environments. Observing a single molecule removes the usual ensemble average, allowing the exploration of hidden heterogeneity in complex condensed phases as well as direct observation of dynamical state changes arising from photophysics and photochemistry, without synchronization. This article… Expand
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