Methods of determining plasma and tissue binding of drugs. Pharmacokinetic consequences.

@article{Pacifici1992MethodsOD,
  title={Methods of determining plasma and tissue binding of drugs. Pharmacokinetic consequences.},
  author={Gian Maria Pacifici and A. Lopez-Duran Viani},
  journal={Clinical pharmacokinetics},
  year={1992},
  volume={23 6},
  pages={449-68}
}
The available techniques for the investigation of drug binding to plasma and tissues protein are reviewed and the advantages and disadvantages of the various techniques stated. A comparison of different plasma protein binding techniques is made which shows that the size of the unbound fraction of drug may be influenced by the method used. Protein binding may be assayed by methods including equilibrium dialysis, ultrafiltration, ultracentrifugation, gel filtration, binding to albumin… CONTINUE READING