Methods of antigen recovery vary in their usefulness in unmasking specific antigens in immunohistochemistry.

  title={Methods of antigen recovery vary in their usefulness in unmasking specific antigens in immunohistochemistry.},
  author={Andra R. Frost and Dorothy Ann Sparks and William E. Grizzle},
  journal={Applied immunohistochemistry & molecular morphology : AIMM},
  volume={8 3},
Microwave heating of histologic sections in citrate buffer (MAR) is a widely used method of antigen recovery but often results in loss of tissue sections. Low-temperature antigen retrieval (LTAR), incubation at 80 degrees C in citrate buffer for 2 hours with trypsin pretreatment is an alternative method reported to result in better antigen recovery for specific antigens as well as decreased loss of tissue sections. To optimize our immunohistochemical evaluation of breast carcinomas, we compared… CONTINUE READING

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