Methods in laboratory investigation. Isolation of guinea pig monocytes and Kurloff cells: characterization of monocyte subsets by morphology, cytochemistry, and adherence.

Abstract

Guinea pig mononuclear cells were separated from peripheral blood using high-density Ficoll-Hypaque, following which the monocytes and Kurloff cells were isolated using counterflow centrifugation elutriation and Percoll gradient centrifugation. Monocytes were obtained in four counterflow centrifugation elutriation fractions. Small monocytes (283 micron3… (More)

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