Methods for using centrifugal elutriation to separate malignant and lymphoid cell populations.

@article{Lord1984MethodsFU,
  title={Methods for using centrifugal elutriation to separate malignant and lymphoid cell populations.},
  author={Edith M. Lord and Peter C. Keng},
  journal={Journal of immunological methods},
  year={1984},
  volume={68 1-2},
  pages={147-55}
}
Malignant cells and non-neoplastic host cells were isolated from in vivo growing solid tumors of 2 BALB/c transplantable tumors: EMT6/Ro, a mammary sarcoma, and Line 1, an alveolar cell carcinoma. The technique of centrifugal elutriation was used to separate these 2 major types of cells present within solid tumors. In addition it was found that 2 successive passages through the elutriator of the cells from the tumors resulted in a better separation of the host cells into lymphocyte and… CONTINUE READING