Methods for the separation and identification of mycobactins from various species of mycobacteria.

  title={Methods for the separation and identification of mycobactins from various species of mycobacteria.},
  author={A. White and G. Snow},
  journal={The Biochemical journal},
  volume={108 4},
A single pure component was isolated from mycobactin P by countercurrent distribution; its side chain is n-cis-octadec-2-enoyl; its purity and molecular structure were confirmed by mass spectrometry of its aluminium complex. The separation of ferric and of aluminium complexes of mycobactins by thin-layer chromatography is described. Mycobacterium terrae, M. marinum and M. smegmatis produce mycobactins that differ among themselves and from mycobactins P and T. A nomenclature for the mycobactins… Expand
Study of Mycobactin From Different Mycobacterial Species by its Effect on Growth of Mycobacterium Avium Subsp Paratuberculosis
Out of all extracted mycobactins used for the growth assay, my- cobactins extracted from M tuberculosis, M phlei and MAP had produced best growth of MAP, indicating its ability to utilize iron efficiently. Expand
Structure of mycobactin J
Mycobactin J-1, an iron chelate fromMycobacterium paratuberculosis, was characterized by mass spectrum and by1H nuclear magnetic resonance (NMR) and13C NMR spectra of the parent molecule and ofExpand
Uptake of salicylic acid into mycobactin S by growing cells of Mycobacterium smegmatis
The formations of the free aromatic acid and mycobactin have both been reported to be stimulated by a deficiency of iron in the growth medium. Expand
A simple and reliable EDDA method for mycobactin production in mycobacteria: Optimal conditions and use in mycobacterial speciation
Ethylenediamino-di-(0-hydroxyphenylacetic acid) (EDDA) was found to be a suitable iron chelator for investigating mycobactin production in mycOBacteria and was applied to representative strains of selected species to yield reliable results useful for identification and speciation ofMycobacteria. Expand
Accumulation of iron-binding phenolic acids by Actinomycetales and other organisms related to the Mycobacteria.
SUMMARY: After growth of representatives of eight genera in the order Actinomycetales and two others related taxonomically to Mycobacterium under iron-deficient conditions, the following compoundsExpand
A review of the methods of identification and differentiation of mycobacteria.
Tests reported to be useful for the differentiation of species or groups of mycobacteria are reviewed and an international system of identification is desirable. Expand
Mycobactins and exochelins of Mycobacterium tuberculosis, M. bovis, M. africanum and other related species.
Following iron-deficient growth, mycobactins and exochelins were isolated from 11 strains of Mycobacterium tuberculosis as well as from M. bovis and M. africanum, supporting previous claims that this group is a single taxon. Expand
Transport of iron by mycobactin in Mycobacterium smegmatis.
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FeCl3 was the more effective source of iron for the cell although Fe-mycobactin was adsorbed on to the cell very quickly and it probably functions as an ionophore for the transport of iron across the lipid envelope of the cell. Expand
Isolation, properties and taxonomic relevance of lipid-soluble, iron-binding compounds (the nocobactins) from Nocardia.
The correlation of the nocobactin data with previous results of conventional numerical taxonomy is high and two strains of N. asteroides, however, did not produce nOCobactins of the group type. Expand
Iron transport in Mycobacterium smegmatis: the role of mycobactin.
A mechanism whereby mycobactin operates as a carrier for iron across the lipid envelope of theMycobacteria is described, and both compounds will be at their highest concentrations at the two lipid-water interfaces of the cell envelope: the outer and inner surfaces. Expand


however (for it was the literal soul of the life of the Redeemer, John xv. io), is the peculiar token of fellowship with the Redeemer. That love to God (what is meant here is not God’s love to men)Expand
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