Methods for studying the binding of aluminum by serum protein.

  title={Methods for studying the binding of aluminum by serum protein.},
  author={Hamidur Rahman and Andrew W. Skillen and S M Channon and Michael K M Ward and David S. Kerr},
  journal={Clinical chemistry},
  volume={31 12},
We describe methods for studying the binding of Al by protein in serum: ultrafiltration, gel filtration, and immuno-affinity chromatography. For ultrafiltration we used an Amicon YM10 cellophane membrane with a nominal cutoff of 10 000 Da to separate ultrafiltrable and non-ultrafiltrable Al. For gel filtration we used Sephacryl S-300, and for immuno-affinity chromatography we used anti-transferrin coupled to CNBr-activated Sepharose to identify the Al-binding protein. For 30 normal subjects 54… CONTINUE READING
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