Methods for studying spindle assembly and chromosome condensation in Xenopus egg extracts.

@article{Maresca2006MethodsFS,
  title={Methods for studying spindle assembly and chromosome condensation in Xenopus egg extracts.},
  author={Thomas J Maresca and Rebecca Heald},
  journal={Methods in molecular biology},
  year={2006},
  volume={322},
  pages={459-74}
}
Methods are presented for preparing cytoplasmic extracts from Xenopus laevis eggs and their utilization to reconstitute and monitor events of the cell cycle in vitro. Addition of sperm nuclei to crude extracts and "cycling" of the reaction through interphase and back into metaphase promotes formation of bipolar spindles capable of segregating their duplicated chromosomes. Reactions can be "spun down" onto cover slips for immunofluorescence analysis. High-speed extracts support mitotic… CONTINUE READING