Methods for meiotic chromosome preparation, immunofluorescence, and fluorescence in situ hybridization in Daphnia pulex.

@article{Tsuchiya2009MethodsFM,
  title={Methods for meiotic chromosome preparation, immunofluorescence, and fluorescence in situ hybridization in Daphnia pulex.},
  author={Dai Tsuchiya and Brian Donovan Eads and Miriam E. Zolan},
  journal={Methods in molecular biology},
  year={2009},
  volume={558},
  pages={235-49}
}
The genus Daphnia has an intriguing reproductive mode of cyclical parthenogenesis. This reproductive mode has been studied for centuries, but cytogenetic information is lacking due to technical limitations of classical methods. We have developed methods for the preparation and examination of meiotic chromosomes of Daphnia pulex from oocytes and spermatocytes. Oocyte chromosome preparations are obtained by isolating individual oocytes after the release of yolk granules from the ovary using… CONTINUE READING