Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria.

  title={Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria.},
  author={Donna M. Penrose and Bernard R. Glick},
  journal={Physiologia plantarum},
  volume={118 1},
One of the major mechanisms utilized by plant growth-promoting rhizobacteria (PGPR) to facilitate plant growth and development is the lowering of ethylene levels by deamination of 1-aminocyclopropane-1-carboxylic acid (ACC) the immediate precursor of ethylene in plants. The enzyme catalysing this reaction, ACC deaminase, hydrolyses ACC to alpha-ketobutyrate and ammonia. Several bacterial strains that can utilize ACC as a sole source of nitrogen have been isolated from rhizosphere soil samples… 

Possible mechanisms for the equilibrium of ACC and role of ACC deaminase-producing bacteria

Proposed candidate attractants involved in the transfer of ACC into ACC deaminase-producing bacteria are proposed for the transfer and equilibrium of exuded ACC under abiotic stress conditions.

Bacterial ACC deaminase and the alleviation of plant stress.

  • B. Glick
  • Biology
    Advances in applied microbiology
  • 2004

Isolation and characterization of 1-aminocyclopropane- 1-carboxylate (ACC) deaminase-containing plant growth-promoting rhizobacteria from carnation soil and roots

It is suggested that ACC deaminase-containing PGPR could be a cost-effective, environment-friendly and promising potential strategy to promote plant growth, alleviate biotic and abiotic stresses and ensure sustainable agriculture, especially for ethylene-sensitive flowers production.

Promoting plant growth using ACC deaminase-producing bacteria: insights into plant-bacterial interactions and agricultural and biotechnological applications

The results obtained in this work demonstrate the important role that the enzyme ACC deaminase plays in the interaction between plant and bacteria, namely in the nodulation process of legumes, and in promoting the growth and development of some plant species.

Methods to Study 1-Aminocyclopropane-1-carboxylate (ACC) Deaminase in Plant Growth-Promoting Bacteria

The result of the functioning of this enzyme is an increase in the growth of plant roots and shoots and a reduction of the inhibitory effects of ethylene synthesis especially during stressful conditions.

Bacterial biosynthesis of 1-aminocyclopropane-1-caboxylate (ACC) deaminase, a useful trait to elongation and endophytic colonization of the roots of rice under constant flooded conditions

Results of this study showed ACC deaminase containing P. fluorescens REN1 increased in vitro root elongation and endophytically colonized the root of rice seedlings significantly, as compared to control under constant flooded conditions, and it may be suggested that the utilization of ACC as a nutrient gives the isolates advantages in more endophytic colonization and increase of root length of Rice seedlings.

Isolation of Rhizobacteria from Jatropha curcas and characterization of produced ACC deaminase

This study first time confirms the ACC deaminase activity in the Enterobacter cancerogenus on the preliminary basis and discusses the possible implications of these bacterial mechanisms on the plant growth promotion or homeostasis regulation in natural conditions.

Characterization of ACC deaminase from the biocontrol and plant growth-promoting agent Trichoderma asperellum T203.

A role for ACCD in the plant root growth-promotion effect by T. asperellum is suggested as well as decreased ability of the mutants to promote root elongation of canola seedlings.

Investigation of ACC-Deaminase and Indole Acetic Acid Producing Bacteria from Rhizospheric Soils in Ağrı Province

Plant growth promoting rhizobacteria (PGPR) have critical role in promoting plant growth and health with various mechanisms. Production of indole acetic acid (IAA) and



Isolation and characterization of ACC deaminase genes from two different plant growth-promoting rhizobacteria.

Southern hybridization experiments suggest that there is a single copy of the ACC deaminase gene in Enterobacter cloacae strains UW4 and CAL2 and that there may be several different types of ACC deaminationase genes in different microbes.

A novel procedure for rapid isolation of plant growth promoting pseudomonads

The method described here may be used to replace the otherwise slow and tedious process of testing individual bacterial strains for their ability to promote plant growth, thereby significantly speeding up the process of finding new PGPR.

Determination of 1-aminocycopropane-1-carboxylic acid (ACC) to assess the effects of ACC deaminase-containing bacteria on roots of canola seedlings.

It was found that, in the presence of the ethylene inhibitor, AVG, or any one of several ACC deaminase-containing strains of bacteria, the growth of canola seedling roots was enhanced and the ACC levels in these roots were lowered.

Partial purification and characterization of 1-aminocyclopropane-1-carboxylate deaminase from the plant growth promoting rhizobacterium Pseudomonas putida GR12-2

The plant growth promoting rhizobacterium Pseudomonas putida GR12-2 can utilize 1-aminocyclopropane-1-carboxylate (ACC) as a sole nitrogen source because it possess the unusual enzyme ACC deaminase,

Effect of transferring 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase genes into Pseudomonas fluorescens strain CHA0 and its gacA derivative CHA96 on their growth-promoting and disease-suppressive capacities.

The results suggest that ACC deaminase activity may have lowered the level of plant ethylene thereby increasing root length, and the role of stress-generated plant Ethylene in susceptibility or resistance depends on the host-pathogen system, and on the experimental conditions used.

Characterization of plant growth promoting rhizobacteria isolated from polluted soils and containing 1-aminocyclopropane-1-carboxylate deaminase.

The results suggest that plant growth promoting rhizobacteria containing ACC deaminase are present in various soils and offer promise as a bacterial inoculum for improvement of plant growth, particularly under unfavourable environmental conditions.

Plant growth responses to bacterization with selected Pseudomonas spp. strains and rhizosphere microbial development in hydroponic cultures

Increases in root and shoot fresh weight were observed when the nutrient solutions used for growth of tomato, cucumber, lettuce, and potato plants were bacterized with plant growth promoting

A Plant Growth-Promoting Bacterium That Decreases Nickel Toxicity in Seedlings

The bacterial plant growth-promoting effect in the presence of nickel was probably not attributable to the reduction of nickel uptake by seedlings, and may reflect the ability of the bacterium to lower the level of stress ethylene induced by the nickel.

Growth promotion of canola (rapeseed) seedlings by a strain of Pseudomonas putida under gnotobiotic conditions

Inoculation of canola seeds with a nitrogen-fixing strain of Pseudomonas putida drastically increased the root length of seedlings grown in sterile growth pouches and added phosphate to the growth solution at similar concentrations stimulated root elongation of inoculated and noninoculated seedlings.