Methods for iPS cell generation for basic research and clinical applications

@article{Mochiduki2012MethodsFI,
  title={Methods for iPS cell generation for basic research and clinical applications},
  author={Yuji Mochiduki and Keisuke Okita},
  journal={Biotechnology Journal},
  year={2012},
  volume={7}
}
The induction of pluripotency can be achieved by forced expression of defined factors in somatic cells. The established cells, termed induced pluripotent stem (iPS) cells, have pluripotency and an infinite capacity for self‐renewal in common with embryonic stem (ES) cells. Patient‐specific iPS cells could be a useful source for drug discovery and cell transplantation therapies; however, the original method for iPS cell generation had several issues that were obstacles to their clinical… 

Generation of Mouse and Human Induced Pluripotent Stem Cells (iPSC) from Primary Somatic Cells

Four detailed protocols are explained, in four detailed protocols, the isolation of mouse and human somatic cells and their reprogramming into iPSC, the so-called induced Pluripotent Stem Cells.

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The research shows that continuous passage increases the efficiency of reprogramming and generates more nearly fully reprogrammed iPSCs efficiently and rapidly, suggesting that full reprograming is a gradual process that does not merely end at the point of the activation of endogenous pluripotency-associated genes.

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The current accomplishments and remaining challenges in the production and use of iPSCs for treatment of neurodegenerative diseases of the brain such as Alzheimer's disease and Parkinson's disease are focused on.

Reduced Immunogenicity of Induced Pluripotent Stem Cells Derived from Sertoli Cells

The findings indicate that early-passage Ser-iPS cells retain some somatic memory of Sertoli cells that impacts on immunogenicity of iPS cells and iPS cell-derived cells in vivo and in vitro, and suggest that immune-privileged SERToli cells might represent a preferred source for iPScell generation, if it comes to the use of i PS cell- derived cells for transplantation.

Modelling human disease with pluripotent stem cells.

The current state of the art with regard to modelling diseases affecting the ectodermal, mesodermal and endodermal lineages is discussed, focussing on studies which have demonstrated a disease phenotype in the tissue of interest.

Xeno-Free Materials for Stabilizing Basic Fibroblast Growth Factor and Enhancing Cell Proliferation in Human Pluripotent Stem Cell Cultures

It is observed that a series of synthetic sulfated/sulfonated polymers and saccharides effectively prevented the reduction of bFGF levels in iPSC culture media during storage at 37 °C and some of the tested materials suppressed heat-induced decline in medium performance and maintained cell proliferation.

[Cardiomyocyte differentiation of individual clones murine induced pluripotent stem cells].

Cardiomyocyte differentiation of certain clones of murine induced pluripotent stem cells (iPS) was estimated and was tenfold more effective in attached EBs.

Embryonic and induced pluripotent stem cell differentiation as a tool in neurobiology

The emerging use of neuronal differentiation systems as a tool for unravelling mechanisms that regulate neuronal development and specification, modelling complex neurological diseases and understanding neuronal dysfunction is discussed.

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