Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity.

@article{Tycko2016MethodsFO,
  title={Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity.},
  author={Josh Tycko and Vic E. Myer and Patrick David Hsu},
  journal={Molecular cell},
  year={2016},
  volume={63 3},
  pages={
          355-70
        }
}
Advances in the development of delivery, repair, and specificity strategies for the CRISPR-Cas9 genome engineering toolbox are helping researchers understand gene function with unprecedented precision and sensitivity. CRISPR-Cas9 also holds enormous therapeutic potential for the treatment of genetic disorders by directly correcting disease-causing mutations. Although the Cas9 protein has been shown to bind and cleave DNA at off-target sites, the field of Cas9 specificity is rapidly progressing… CONTINUE READING
BETA

Figures, Tables, and Topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-10 OF 60 CITATIONS

2018-1205 NmeCas 9 is an intrinsically high-fidelity genome-editing platform

VIEW 7 EXCERPTS
CITES METHODS, BACKGROUND & RESULTS
HIGHLY INFLUENCED

NmeCas 9 is an intrinsically high-fidelity genome editing platform 2 3 4 5

VIEW 8 EXCERPTS
CITES BACKGROUND, RESULTS & METHODS
HIGHLY INFLUENCED

NmeCas9 is an intrinsically high-fidelity genome-editing platform

  • Genome Biology
  • 2018
VIEW 7 EXCERPTS
CITES METHODS, BACKGROUND & RESULTS
HIGHLY INFLUENCED

Potential high-frequency off-target mutagenesis induced by CRISPR/Cas9 in Arabidopsis and its prevention

  • Plant Molecular Biology
  • 2018
VIEW 5 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

FILTER CITATIONS BY YEAR

2016
2019

CITATION STATISTICS

  • 8 Highly Influenced Citations

  • Averaged 18 Citations per year over the last 3 years

References

Publications referenced by this paper.
SHOWING 1-10 OF 159 REFERENCES

CRISPR-Cas9 nucleases with altered PAM specificities

B. P. Kleinstiver, V. Pattanayak, +4 authors J. K. Joung
  • Nature 529,
  • 2016
VIEW 18 EXCERPTS
HIGHLY INFLUENTIAL

Streptococcus thermophilus CRISPR-Cas9 Systems Enable Specific Editing of the Human Genome.

  • Molecular therapy : the journal of the American Society of Gene Therapy
  • 2016
VIEW 4 EXCERPTS
HIGHLY INFLUENTIAL

In vivo genome editing using Staphylococcus aureus Cas9

  • Nature
  • 2015
VIEW 25 EXCERPTS
HIGHLY INFLUENTIAL

CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors

A. V. Wright, J. K. Nuñez, J. A. Doudna
  • Nat. Biotechnol
  • 2016
VIEW 4 EXCERPTS
HIGHLY INFLUENTIAL

Similar Papers

Loading similar papers…