Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity.

@article{Tycko2016MethodsFO,
  title={Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity.},
  author={Josh Tycko and Vic E. Myer and Patrick David Hsu},
  journal={Molecular cell},
  year={2016},
  volume={63 3},
  pages={
          355-70
        }
}
Advances in the development of delivery, repair, and specificity strategies for the CRISPR-Cas9 genome engineering toolbox are helping researchers understand gene function with unprecedented precision and sensitivity. CRISPR-Cas9 also holds enormous therapeutic potential for the treatment of genetic disorders by directly correcting disease-causing mutations. Although the Cas9 protein has been shown to bind and cleave DNA at off-target sites, the field of Cas9 specificity is rapidly progressing… CONTINUE READING
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