Methods and Applications of CRISPR-Mediated Base Editing in Eukaryotic Genomes.

@article{Hess2017MethodsAA,
  title={Methods and Applications of CRISPR-Mediated Base Editing in Eukaryotic Genomes.},
  author={Gaelen T. Hess and Josh Tycko and David H. Yao and Michael C Bassik},
  journal={Molecular cell},
  year={2017},
  volume={68 1},
  pages={
          26-43
        }
}
The past several years have seen an explosion in development of applications for the CRISPR-Cas9 system, from efficient genome editing, to high-throughput screening, to recruitment of a range of DNA and chromatin-modifying enzymes. While homology-directed repair (HDR) coupled with Cas9 nuclease cleavage has been used with great success to repair and re-write genomes, recently developed base-editing systems present a useful orthogonal strategy to engineer nucleotide substitutions. Base editing… CONTINUE READING
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