Methodology of protistan discovery: from rRNA detection to quality scanning electron microscope images.

@article{Stoeck2003MethodologyOP,
  title={Methodology of protistan discovery: from rRNA detection to quality scanning electron microscope images.},
  author={Thorsten Stoeck and William H Fowle and Slava S. Epstein},
  journal={Applied and environmental microbiology},
  year={2003},
  volume={69 11},
  pages={6856-63}
}
Each year, thousands of new protistan 18S rRNA sequences are detected in environmental samples. Many of these sequences are molecular signatures of new protistan species, classes, and/or kingdoms that have never been seen before. The main goal of this study was to enable visualization of these novel organisms and to conduct quality ultrastructural examination. We achieved this goal by modifying standard procedures for cell fixation, fluorescence in situ hybridization, and scanning electron… CONTINUE READING

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