Methodology in experimental infections of mice with Hymenolepis nana

  title={Methodology in experimental infections of mice with Hymenolepis nana},
  author={G. Ferretti and F. Gabriele and C. Palmas},
  journal={Italian Journal of Zoology},
Abstract A “natural” infection technique based on the uptake of eggs with feed is described. The ratio of the number of parasites recovered 14 days post infection to the number of eggs given (q ratio) is close to 1 if few eggs are given (⋍ 5 per mouse). In the same conditions, q is less in mice infected with ⋍ 50 or ⋍ 500 eggs. The larger the number of parasites, the faster their spontaneous elimination. Total elimination or “self-cure” may be attained even in the case of large numbers. In mice… Expand
Susceptibility of different strains of mice to various levels of infection with the eggs of Taenia taeniaeformis.
It is proposed that in resistant strains, infection with 30–50 eggs induces a more rapid immune response which becomes effective while the larva is still vulnerable; in massive infections, however, immune paralysis may occur. Expand
Development of human and mouse strain of Hymenolepis nana in mice.
Discussion bears on the hypothesis that the difficulties that emerge during the first passages may be connected to the adult and not to the larval phase, and appears the same in the 3 parasite strains. Expand
Interactions between Trichinella spiralis and Hymenolepis nana in the intestine of the mouse.
The rejection of the intestinal phase of Trichinella spiralis in outbred CD 1 mice is associated with an inflammatory response and the distribution of parasites suggests that immunodepression is involved. Expand
Specific cross-immunity between Hymenolepis nana and H. diminuta: immunization with heterologous and homologous light infections.
The reciprocal cross immunity between the heterologous parasites and the failure of protection of homologous challenges are discussed in relation to light infections. Expand
The therapeutic effect of tamoxifen drug on mice experimentally infected with H. nana did not show statisticallysignificant effect on worm burden as compared to control one, with the exception of micetreated two days post-infection that showed significant increase in worm burden. Expand
Immune response to the cestode Hymenolepis nana: cytokine production during infection with eggs or cysts.
The level of IFN-gamma paralleled that seen after a primary egg infection and suggests that there is a predominantly Th1-type response during the tissue phase of H. nana development and that, in BALB/c mice, a Th2 polarization occurs during the first few days of the lumenal phase. Expand
Blast cell activity in mice infected with Hymenolepis nana, H. diminuta and Trichinella spiralis: in vivo uptake of 125IUdR in lymphoid tissues and gut.
It has been shown that the increase in lymphoblast activity was closely correlated with the presence of cells which are most effective in adoptive transfer immunity. Expand
Failure to infect laboratory rodent hosts with human isolates of Rodentolepis (= Hymenolepis) nana
The large sample size used in this study, and the range of techniques employed for extraction and preparation of eggs provide a comprehensive test of the hypothesis that the human strain of R. nana is essentially non-infective to rodents. Expand
Causality or coincidence: may the slow disappearance of helminths be responsible for the imbalances in immune control mechanisms?
It has been suggested that Th2 responses can make the host more susceptible to other important diseases and to contribute to the spread of them, and it is suggested that failure to acquire worms and experience mucosal Th2 conditioning predisposes to unrelated diseases. Expand
Immunological memory and lymphoblast-migration in mice infected withHymenolepis nana
Preliminary evidence for the existence of T memory cells in the spleen and in the mesenteric nodes is provided for the first time in mice inbred with the intestinal parasite Hymenolepis nana. Expand


The mode of passive protection against Hymenolepis nana induced by serum transfer.
  • A. Ito
  • Biology, Medicine
  • International journal for parasitology
  • 1977
A protective immunity against the cestode Hymenolepis nana was transferred with serum taken from actively immunized mice, and the major effect of immune serum was damaging hatched oncospheres in both the intestinal lumen and the villi within 1 day post infection. Expand
Population dynamics of Hymenolepis nana in mice: fecundity and the 'crowding effect'.
Young white mice were infected with different doses of eggs or cysticercoids of Hymenolepis nana and the subsequent faecal egg output was measured, correlated with worm numbers, determined after killing the hosts. Expand
Studies on helminth immunity. IV. Rapid onset of resistance by the white mouse against a challenging infection with eggs of Hymenolepis nana (Cestoda: Hymenolepididae).
It is concluded that a local, rapidly mobilized antibody reaction by the immediate cells affected could be responsible in spite of the brief period involved, and this would provide interesting evidence supporting the concept that locally concentrated cellular antibodies are an important host response to tissue invasion by helminths. Expand
The mechanism of hatching of eggs of Haemonchus contortus.
The ‘spontaneous’ hatching of eggs of H. contortus was strongly inhibited by 1,10-phenanthroline, 10 −3 M, and this inhibition was reversed by Zn 2+ , but the inhibition of ‘hatching’ of eggs in externally applied hatching fluid, or the hydrolysis of leucinamide in hatch fluid was generally less marked. Expand
A probabilistic approach to the statistical analysis of experiments on helminth parasites.
A probabilistic method, based on a Bayesian approach, is proposed for the evaluation of results obtained in experimental infections induced by microscopic eggs (or larvae) whose exact number is not directly known; the results are represented by normalized likelihood functions (NLF) or, more generally, through final probability distributions. Expand
Hymenolepis diminuta: ultrastructural localization of immunoglobulin-binding sites on the tegument.
Abstract Horseradish peroxidase-conjugated antiserum was used to investigate with the electron microscope the earlier report that host IgA, IgM, IgG, and complement component 3 occur on HymenolepisExpand