Methodology for Spot Quality Evaluation Semi-automatic Pipeline in Maia

    The general workflow of the semi-automatic pipeline analysis in MAIA is shown in Figure 1A, Manuscript. In Block 1 raw data, i.e. .tif and .gal (GenePix Array List) files, are imported in the program. The automatic image analysis consists of Spot Localization (Block 2), Image Alignment (Block 3), Spot Quantification (Block 4) and Quality Analysis (Block 5… CONTINUE READING