Methodologies and their limitations

  title={Methodologies and their limitations},
  author={Edward John Thompson},
This chapter discusses steps in the determination of protein levels. This involves separation, visualization, and estimation. Ideally, cerebrospinal fluid (CSF) proteins should first be separated on the basis of their size and/or charge. The most discriminating method of separation is electrophoresis on polyacrylamide gels, where the sieving effect of acrylamide allows discrimination on the basis of molecular size in addition to the molecular charge. They should then be visualized and finally… CONTINUE READING