Methodological considerations in estrogen assays of breast fluid and breast tissue

@article{Chatterton2015MethodologicalCI,
  title={Methodological considerations in estrogen assays of breast fluid and breast tissue},
  author={R. Chatterton and M. Muzzio and R. Heinz and P. Gann and Seema A. Khan},
  journal={Steroids},
  year={2015},
  volume={99},
  pages={103-107}
}
Estradiol (E2) in nipple aspirate fluid (NAF), ductal lavage fluid (DLF), and random fine needle aspirates (rFNA) are compared. Quantification was by immunoassay or tandem MS. The percent of women yielding NAF varied between 24% and 48% and for DLF was 86.3%. Variation between ducts within a breast was not less than variation between breasts within women but variation between breasts and within women over time was significantly less than variation between women. Serum E2 was highly… Expand
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  • 2014
We thank Drs. Chatterton, Muzzio, and Gann for their comments regarding the methods that were used to determine estrogens in nipple aspirate fluid (NAF) and ductal lavage supernatant (DLS) [1]. Dr.Expand
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Circulating estrogens and estrogens within the breast among postmenopausal BRCA1/2 mutation carriers
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It is demonstrated that circulating PE and EM are strongly and positively correlated with tissue-specific PE andEM measured in NAF and DLS among postmenopausal BRCA1/2 mutation carriers, and future etiologic studies could utilize the more readily obtainable serum hormone levels as a reliable surrogate measure of exposure at the tissue level. Expand
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