Methodological considerations for using umu assay to assess photo-genotoxicity of engineered nanoparticles.

@article{Cupi2016MethodologicalCF,
  title={Methodological considerations for using umu assay to assess photo-genotoxicity of engineered nanoparticles.},
  author={Denisa Cupi and Anders Baun},
  journal={Mutation research. Genetic toxicology and environmental mutagenesis},
  year={2016},
  volume={796},
  pages={
          34-9
        }
}
In this study we investigated the feasibility of high-throughput (96-well plate) umu assay to test the genotoxic effect of TiO2 engineered nanoparticles (ENPs) under UV light (full spectrum) and visible light (455 nm). Exposure of TiO2 ENPs to up to 60 min of UV light induced a photocatalytic production of ROS. However, UV light itself caused cytotoxic damage to Salmonella typhimurium at exposures >15 min and a genotoxic effect at exposures >0.5 min; and use of UV filters did not lower this… CONTINUE READING
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