Methodological Considerations for the Use of Stable Isotope Probing in Microbial Ecology

@article{Neufeld2006MethodologicalCF,
  title={Methodological Considerations for the Use of Stable Isotope Probing in Microbial Ecology},
  author={Josh D. Neufeld and Marc G. Dumont and Jyotsna Vohra and John Colin Murrell},
  journal={Microbial Ecology},
  year={2006},
  volume={53},
  pages={435-442}
}
Stable isotope probing (SIP) is a method used for labeling uncultivated microorganisms in environmental samples or directly in field studies using substrate enriched with stable isotope (e.g., 13C). After consumption of the substrate, the cells of microorganisms that consumed the substrate become enriched in the isotope. Labeled biomarkers, such as phospholipid-derived fatty acid (PLFA), ribosomal RNA, and DNA can be analyzed with a range of molecular and analytical techniques, and used to… CONTINUE READING
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