Method to convert N-terminal glutamine to pyroglutamate for characterization of recombinant monoclonal antibodies.

@article{Xu2013MethodTC,
  title={Method to convert N-terminal glutamine to pyroglutamate for characterization of recombinant monoclonal antibodies.},
  author={Wei Xu and Yan Peng and Fengqiang Wang and Brittany Paporello and Daisy Richardson and Hongcheng Liu},
  journal={Analytical biochemistry},
  year={2013},
  volume={436 1},
  pages={10-2}
}
Cyclization of N-terminal glutamine to pyroglutamate is a common modification of recombinant monoclonal antibodies that has often been identified by liquid chromatography mass spectrometry (LC-MS) analysis using separated fractions. An alternative approach of using glutaminyl-peptide cyclotransferase to convert the N-terminal glutamine to pyroglutamate was developed in the current study. Enzymatic conversion of the N-terminal glutamine to pyroglutamate not only provides an identification of the… CONTINUE READING

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