Method of specific detection of apoptosis using formamide-induced DNA denaturation assay.

@article{Ito2006MethodOS,
  title={Method of specific detection of apoptosis using formamide-induced DNA denaturation assay.},
  author={Yuko Ito and Masa-Aki Shibata and Ken Takeshi Kusakabe and Yoshinori Otsuki},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={2006},
  volume={54 6},
  pages={683-92}
}
We compared the reliability between apoptosis detection methods, namely, the terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) method and formamide-induced DNA denaturation assay using a monoclonal antibody (MAb) to single-stranded DNA (ssDNA) (formamide-MAb assay). Reaction targets in these methods are different: the TUNEL method recognizes free 3'-OH DNA ends, whereas the formamide-MAb assay detects ssDNA itself (25-30 bp). We found that the formamide… CONTINUE READING