Method of oocyte activation affects cloning efficiency in pigs.

@article{Whitworth2009MethodOO,
  title={Method of oocyte activation affects cloning efficiency in pigs.},
  author={Kristin M Whitworth and Rongfeng Li and Lee D. Spate and David M. Wax and August Rieke and Jeffrey J. Whyte and Gaurishankar Manandhar and Miriam Sutovsky and Jonathan Alexander Green and Peter Sutovsky and Randall S Prather},
  journal={Molecular reproduction and development},
  year={2009},
  volume={76 5},
  pages={
          490-500
        }
}
The following experiments compared the efficiency of three fusion/activation protocols following somatic cell nuclear transfer (SCNT) with porcine somatic cells transfected with enhanced green fluorescent protein driven by the chicken beta-actin/rabbit beta-globin hybrid promoter (pCAGG-EGFP). The three protocols included electrical fusion/activation (NT1), electrical fusion/activation followed by treatment with a reversible proteasomal inhibitor MG132 (NT2) and electrical fusion in low Ca(2… CONTINUE READING
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