Method for the generation and cultivation of functional three-dimensional mammary constructs without exogenous extracellular matrix

Abstract

During puberty, pregnancy, lactation and post-lactation, breast tissue undergoes extensive remodelling and the disruption of these events can lead to cancer. In vitro studies of mammary tissue and its malignant transformation regularly employ mammary epithelial cells cultivated on matrigel or floating collagen rafts. In these cultures, mammary epithelial… (More)
DOI: 10.1007/s00441-004-1064-6

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