Method for the determination of vitamin K homologues in human plasma using high-performance liquid chromatography-tandem mass spectrometry.

@article{Suhara2005MethodFT,
  title={Method for the determination of vitamin K homologues in human plasma using high-performance liquid chromatography-tandem mass spectrometry.},
  author={Yoshitomo Suhara and Maya Kamao and Naoko Tsugawa and Toshio Okano},
  journal={Analytical chemistry},
  year={2005},
  volume={77 3},
  pages={757-63}
}
We report here the development of a precise and sensitive method for the determination of vitamin K homologues including phylloquinone (PK), menaquinone-4 (MK-4), and menaquinone-7 (MK-7) in human plasma using HPLC-tandem mass-mass spectrometry with atmospheric pressure chemical ionization (LC-APCI-MS/MS). The method involves the use of stable isotope (18)O-labeled internal standard compounds, which were synthesized in our laboratory, and the selection of a precursor and product ion with a MS… CONTINUE READING