Method for the detection and quantitation of chitinase inhibitors in fermentation broths; isolation and insect life cycle effect of A82516.

@article{Somers1987MethodFT,
  title={Method for the detection and quantitation of chitinase inhibitors in fermentation broths; isolation and insect life cycle effect of A82516.},
  author={Peter J. Somers and Raymond Che-Fong Yao and Lawrence E. Doolin and Michael J. McGowan and David S. Fukuda and Jon S. Mynderse},
  journal={The Journal of antibiotics},
  year={1987},
  volume={40 12},
  pages={
          1751-6
        }
}
A new method of screening for chitinase inhibitors in crude fermentation broths as a means of discovering new insecticidal leads has been developed. In this procedure soluble Remazol brilliant violet 5R dye-coupled chitin degradation products released from insoluble chitin azure substrate by hydrolysis with Streptomyces griseus chitinase are filtered in 0.45 micron Millititer HA 96 well filtration plates and collected in 96 well microtiter plates. Inhibitors of this reaction are detected by a… 

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