Method for the detection and quantitation of chitinase inhibitors in fermentation broths; isolation and insect life cycle effect of A82516.

@article{Somers1987MethodFT,
  title={Method for the detection and quantitation of chitinase inhibitors in fermentation broths; isolation and insect life cycle effect of A82516.},
  author={Peter J. Somers and Raymond Che-Fong Yao and Lawrence E. Doolin and Michael J. McGowan and David S. Fukuda and Jon S. Mynderse},
  journal={The Journal of antibiotics},
  year={1987},
  volume={40 12},
  pages={
          1751-6
        }
}
A new method of screening for chitinase inhibitors in crude fermentation broths as a means of discovering new insecticidal leads has been developed. In this procedure soluble Remazol brilliant violet 5R dye-coupled chitin degradation products released from insoluble chitin azure substrate by hydrolysis with Streptomyces griseus chitinase are filtered in 0.45 micron Millititer HA 96 well filtration plates and collected in 96 well microtiter plates. Inhibitors of this reaction are detected by a… 
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The complete and exclusive inhibition of N- acetyl-β-D-glucosaminidase by an acetylgluconolactone-derivative and chitinase activity by allosamidin indicates that both enzymes can be determined separately in crude extracts, with the substrates 4-methylumbelliferyl-N-acetylglucOSaminide or 4- methylumbellIFeryl-chitoriose, respectively.
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Functional expression of the Spodoptera exigua chitinase to examine the virtually screened inhibitor candidates
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New information for the chitinase of beet armyworm and for chit inase inhibitor development is provided for the first time.
Detection and characterization of chitinases and other chitin-modifying enzymes
TLDR
This review summarizes many methods that can be used to isolate and characterize chitin-modifying enzymes including chit in depolymerases, chitodextrinases, Chitin deacetylases, N-acetylglucosaminidase, ch itin-binding proteins, and chitosanases.
Expression and Secretion of a Larval-specific Chitinase (Family 18 Glycosyl Hydrolase) by the Infective Stages of the Parasitic Nematode, Onchocerca volvulus *
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It is concluded that Ov-CHI-1 is a highly stage-specific enzyme that may have a role in infectivity of the parasite, aiding escape from the vector or participating in early post-infective migration and/or development.
Inhibitors of chitinases.
TLDR
There is a pronounced species specificity in chitinase inhibition by allosamidin: half-maximal values are often in the range of 0.1-1 microM (e.g. in all arthropods), being lower in nematodes and amoeba and quite divergent in fungi (0.01-70 microM).
Chemical modification studies of the active centre of Candida albicans chitinase and its inhibition by allosamidin.
TLDR
Kinetic studies of this inactivation provided evidence for the formation of a reversible complex between allosamidin and chitinase, and chemical modification studies with the use of group-specific reagents suggested the presence of Glu/Asp carboxyl group(s) at or near the active site, that were important for enzyme activity.
Detection of novel secondary metabolites.
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This review focuses on the genesis and development of the gamut of methodologies that have led to the successful detection of the wide variety of novel secondary metabolites that include antibacterial, antigungal, antiviral and antitumour antibiotics, enzyme inhibitors, pharmacologically and immunologically active agents, products useful in agriculture and animal husbandry, microbial regulators, and other compounds for which no bioactive role has yet been found.
Inhibition of chitinolytic activities from tree species and associated fungi.
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