Method for recovery of intact DNA for community analysis of marine intertidal microbial biofilms.

@article{NarvezZapata2005MethodFR,
  title={Method for recovery of intact DNA for community analysis of marine intertidal microbial biofilms.},
  author={Jos{\'e} A Narv{\'a}ez-Zapata and Norma Laura Rodr{\'i}guez-{\'A}vila and Benjam{\'i}n Otto Ortega-Morales},
  journal={Molecular biotechnology},
  year={2005},
  volume={30 1},
  pages={51-6}
}
A protocol is described for rapid DNA isolation from marine biofilm microorganisms embedded in large amounts of exopolysaccharides. The method is a modification of the hot phenol protocol used for plants tissues, where nonexpensive and easily available enzymes were used. The method is based on the incubation of biofilm biomass samples in an extraction buffer mixed with phenol preheated at 65 degrees C. The procedure can be completed in 2 h and up to 20 samples can be processed simultaneously… CONTINUE READING