Method for measuring dissolved hydrogen in anaerobic ecosystems: application to the rumen.

@article{Robinson1981MethodFM,
  title={Method for measuring dissolved hydrogen in anaerobic ecosystems: application to the rumen.},
  author={Joseph Arlen Robinson and Richard F. Strayer and James M. Tiedje},
  journal={Applied and environmental microbiology},
  year={1981},
  volume={41 2},
  pages={
          545-8
        }
}
A method of transferring dissolved H2 to a CO2 headspace and then absorbing out the CO2 to concentrate the H2 before gas chromatographic analysis was developed to measure low concentrations of dissolved H2. A detection limit of 10 pmol of H2 ml-1 of water was achieved. When used ot monitor H2 changes in a bovine rumen, a 10-fold increase in H2 was noted 1 h after feeding and then declined rapidly to the normal steady-state concentration of 1 microM. 

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