Method for determining whether a gene of Escherichia coli is essential: application to the polA gene.

@article{Joyce1984MethodFD,
  title={Method for determining whether a gene of Escherichia coli is essential: application to the polA gene.},
  author={Catherine Marie Joyce and Nigel D. F. Grindley},
  journal={Journal of bacteriology},
  year={1984},
  volume={158 2},
  pages={
          636-43
        }
}
We have developed a general method for determining whether a gene of Escherichia coli is essential for viability. The method requires cloned DNA spanning the gene in question and a reasonably detailed genetic and physical map of the cloned segment. Using this information, one constructs a deletion of the target gene in vitro. For convenience, the deletion can be marked by an antibiotic resistance gene. A DNA segment containing the deletion is then cloned onto an att delta phage lambda vector… CONTINUE READING

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