Method for determination of free intracellular and extracellular methylglyoxal in animal cells grown in culture.

@article{Chaplen1996MethodFD,
  title={Method for determination of free intracellular and extracellular methylglyoxal in animal cells grown in culture.},
  author={Frank W. R. Chaplen and William E Fahl and Douglas C. Cameron},
  journal={Analytical biochemistry},
  year={1996},
  volume={238 2},
  pages={171-8}
}
Methylglyoxal is present at low levels in most cells as a by-product of glycolysis and a product of lipid and amino acid catabolism. The most widely accepted method for measurement of methylglyoxal involves the derivatization of methylglyoxal with 1,2-diaminobenzene derivatives, such as o-phenylenediamine, followed by quantification of the resulting quinoxaline with high-performance liquid chromatography (HPLC). Here we describe the modification of this procedure for the measurement of free… CONTINUE READING