Methionyl-tRNA synthetase-induced conformational change of Escherichia coli tRNAfMet.

@article{YamashiroMatsumura1981MethionyltRNASC,
  title={Methionyl-tRNA synthetase-induced conformational change of Escherichia coli tRNAfMet.},
  author={S Yamashiro-Matsumura and Mitsuhiro Kawata},
  journal={The Journal of biological chemistry},
  year={1981},
  volume={256 17},
  pages={9308-12}
}
Ribonuclease T2, nuclease S1, and snake venom phosphodiesterase were used as a structural probe for investigation of the interaction between Escherichia coli tRNAfMet and methionyl-tRNA synthetase, and the cleavage sites were analyzed by a rapid sequencing gel electrophoresis of 5'-32P-labeled tRNA. Both endonucleases cleaved the D-loop of synthetase-bound… CONTINUE READING